Xianchan Li

 Xianchan Li CV

xianchan.li@chem.gu.se

 

 

 

Room 4077
Kemivägen 10, Gothenburg

 

Current position
2015.04-present   Researcher, Department of Chemistry and Molecular Biology, University of Gothenburg, Sweden

 

Previous positions and education
2013.04-2015.04   Postdoc, Chalmers University of Technology, Gothenburg, Sweden (Supervisor: Prof. Andrew G. Ewing)
2009.07-2013.04   Assistant Professor, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing, China (Supervisor: Prof. Fuyi Wang)
2004.09-2009.06   Ph.D. in Chemistry, Institute of Chemistry, CAS, Beijing, China (Supervisor: Prof. Lanqun Mao)

 

Research
Neurons communicate through the release of chemical messengers (e.g., neurotransmitters, neurohormones, and neuropeptides), which are packaged intracellularly in submicrometer sized structures called secretory vesicles. During exocytosis, the fundamental method for neurotransmission, vesicles migrate to the plasma membrane of a cell, fuse, and release their contents into the extracellular space. These messengers can then bind to receptors on a target cell, thus inducing a cascade of signaling events in a complex network. The overall goal of my research is to elucidate molecular events on a subcellular level during cell communication with electrochemical method and secondary-ion mass spectrometry to understand mechanism of learning and memory, drug abuse effects, as well as neurodegenerative diseases like Parkinson´s disease.

Selected publications:

  1. X. Li, J. Dunevall, L. Ren, A. G. Ewing*, Mechanistic Aspects of Vesicle Opening during Analysis with Vesicle Impact Electrochemical Cytometry, Anal. Chem. 2017, In press, DOI: 10.1021/acs.analchem.7b02226.
  2. N. T.N. Phan, X. Li, A. G. Ewing*, Measuring synaptic vesicles using cellular electrochemistry and nanoscale molecular imaging, Nature Reviews Chemistry,  0048 (2017) DOI:10.1038/s41570-017-0048.
  3. L. Ren, M. D. Pour, S. Majdi, X. Li, P. Malmberg, A. G. Ewing*. Zinc Regulates Chemical Tranmitter Storage in Nanometer Vesicles and Exocytosis Dynamics Measured by Amperometry. Angew. Chem. Int. Ed. 2017, 56, 4970-4975.
  4. X. Li†, J. Dunevall†, A. G. Ewing*. Quantitative Chemical Measurements of Vesicular Transmitters with Electrochemical Cytometry. Acc. Chem. Res. 2016, 49, 2347-2354.
  5. X. Li, J. Dunevall, A. G. Ewing*. Using Single-Cell Amperometry to Reveal How Cisplatin Treatment Modulates the Release of Catecholamine Transmitters during Exocytosis. Angew. Chem. Int. Ed. 2016, 55, 9041-9044. Back cover: Angew. Chem. Int. Ed. 2016, 55, 9098.
  6. X. Li†, S. Majdi†, J. Dunevall, H. Fathali, A. G. Ewing*. Quantitative Measurement of Transmitters in Individual Vesicles in the Cytoplasm of Single Cells with Nanotip Electrodes. Angew. Chem. Int. Ed. 2015, 54, 11978-11982.
  7. X. Li, A. S. Mohammadi, A. G. Ewing*. Single Cell Amperometry Reveals Curcuminoids Modulate the Release of Neurotransmitters during Exocytosis from PC12 Cells. J. Electroanal. Chem. 2016, 781, 30-35.
  8. S. Majdi, L. Ren, H. Fathali, X. Li, Andrew G. Ewing*. Selected Recent in vivo Studies on Chemical Measurements in Invertebrates. Analyst 2015, 140, 3676-3686.
  9. J. Lovric, X. Li, A. G. Ewing. Quantitative Chemical Measurements of Vesicular Transmitters with Single Cell Amperometry and Electrochemical Cytometry, Chapter 8, in “Compendium of In-Vivo Monitoring in Real-Time Molecular Neuroscience Volume 1,” World Scientific Publishing, (2015). (book chapter)
  10. J. Lovric, J. D. Keighron, T. B. Angerer, X. Li, P. Malmberg, J. S. Fletcher, A. G. Ewing*. Analysis of Liposome Model Systems by Time-of-Flight Secondary Ion Mass Spectrometry. Surf. Interface Anal. 2014, 46, 74-78.
  11. Z. Du, Q. Luo, L. Yang, T. Bing, X. Li, W. Guo, K. Wu, Y. Zhao, S. Xiong, D. Shangguan, F. Wang*. Mass Spectrometric Proteomics Reveals that Nuclear Protein Positive Cofactor PC4 Selectively Binds to Cross-Linked DNA by a trans-Platinum Anticancer Complex. J. Am. Chem. Soc. 2014, 136, 2948–2951.
  12. W. Guo, W. Zheng, Q. Luo, X. Li, Y. Zhao, S. Xiong, and F. Wang*. Transferrin Serves As a Mediator to Deliver Organometallic Ruthenium(II) Anticancer Complexes into Cells. Inorg. Chem. 2013, 52, 5328–5338.
  13. X. Li, P. Yu, L. Yang, F. Wang, L. Mao*. An Electrochemical Method for Investigation of Conformational Flexibility of Active Sites of Trametes versicolor Laccase Based on Sensitive Determination of Copper Ion with Cysteine-Modified Electrodes. Anal. Chem. 2012, 84, 9416-9421.
  14. X. Li, L. Zhao, Z. Chen, Y. Lin, P. Yu, L. Mao*. Continuous Electrochemical Monitoring of Extracellular Lactate Production from Neonatal Rat Cardiomyocytes following Myocardial Hypoxia. Anal. Chem. 2012, 84, 5285-5291.
  15. L. Zhao, X. Li, Y. Lin, L. Yang, P. Yu, L. Mao*. Electrochemical Impedance Spectroscopic Measurements of FCCP-Induced Change in Membrane Permeability of MDCK Cells. Analyst 2012, 137, 2199-2204.
  16. S. Lv, Q. Luo, X. Li, J. Wu, Y. Feng, F.Wang*. Inhibitor Screening of Protein Kinases Using MALDI-TOF MS Combined with Separation and Enrichment of Phosphopeptides by TiO2 Nanoparticle Deposited Capillary Column. Analyst 2010, 135, 2858-2863.
  17. X. Li, W. Zheng, L. Zhang, P. Yu, Y. Lin, L. Su, L. Mao*. Effective Electrochemical Method for Investigation of Hemoglobin Unfolding Based on the Redox Property of Heme Groups at Glassy Carbon Electrodes. Anal. Chem. 2009, 81, 8557-8563.
  18. X. Li, L. Zhang, L. Su, T. Ohsaka, L. Mao*. A Miniature Glucose/O2 Biofuel Cell with a High Tolerance against Ascorbic acid. Fuel Cells 2009, 9, 85-91.
  19. X. Li, H. Zhou, P. Yu, L. Su, T. Ohsaka, L. Mao*. A Miniature Glucose/O2 Biofuel Cell with Single-Walled Carbon Nanotubes-Modified Carbon Fiber Microelectrodes as the Substrate. Electrochem. Commun. 2008, 10, 851-854.
  20. Y. Lu, X. Li, L. Zhang, P. Yu, L. Su, L. Mao*. Aptamer-Based Electrochemical Sensors with Aptamer-Complementary DNA Oligonucleotides as Probe. Anal. Chem. 2008, 80, 1883-1890.